We describe a new type of surface imprinting technique that combines the advantages of both the semi-covalent approach and the one stage miniemulsion polymerization. This process has been successfully applied for the preparation of glucose surface imprinted nanoparticles. The selective artificial receptors for glucopyranoside have been fully characterized by IR, TEM, BET analysis, and their molecular recognition abilities, by binding experiments, carried out in the batch process. The glucose molecularly imprinted polymers prepared in such a way, have been studied and evaluated in order to accurately quantify their molecular affinity and their selectivity. These characteristics are essential for the verification of the efficiency of the developed surface imprinting process.
